This antibody has been shown to work in applications such as: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Find antibodies and reagents all backed by our Guarantee+. view now. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκB-α and p27. Although the importance of modifications of PCNA to DDT pathways is well known, the relevance of its homo-trimer form, in which three K164 residues are present in a single ring, remains to be elucidated. Clonality. PCNA is mono-ubiquitinated through RAD6 and RAD18, modified by lysine-63-linked multi-ubiquitination—which additionally requires MMS2, UBC13 and RAD5—and is conjugated to SUMO by UBC9. health products, bulky and/or heavy items. Monoclonal Antibody Trial Size Samples CLICK HERE FOR A FREE SAMPLE . Global antibody supplier and research reagent supplier to the life science community. Resuspend the cells by adding 2 mL of Flow Cytometry ⦠Formats Unconjugated. An isopeptide bond is formed between the carboxyl group (COO ) of the ubiquitin's glycine and the epsilon-amino group(ε-NH 3) of the substrate's lysine. 37 Full PDFs related to this paper. Modification of proteins by the addition of lysine (K)-63-linked polyubiquitin (polyUb) chains is suggested to play important roles in a variety of cellular events, including DNA repair, signal transduction, and receptor endocytosis. With myc antibody the ubiquitin modified protein might remain undetected because you are mostly detecting the non-modified form. Perform a Western blot using an anti-Ubiquitin antibody. FEATURED APPLICATIONS . Clone FK1 recognises only polyubiquitinylated proteins and not monoubiquitinylated proteins or free ubiquitin, whilst clone FK2 recognises both mono- and poly-ubiquitinylated species but not free ubiquitin. The primary function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S Proteosome. Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. SCBT is a leading producer of monoclonal antibodies, RNAi, CRISPR KO/Activation products and chemicals for research. Alaska, Hawaii, Canada and Puerto Rico. Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. See our complete line of primary antibodies for mammalian and non-mammalian targets. Background The cell adhesion molecule IGPR-1 regulates various critical cellular processes including, cellâcell adhesion, mechanosensing and autophagy and plays important roles Ubiquitin binding modulates IAP antagonist-stimulated proteasomal degradation of c-IAP1 and c-IAP21. Isotype IgG1, kappa. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The Biocompare Antibody Search Tool lets researchers search over 3 million antibodies from hundreds of antibody suppliers quickly and easily. The ubiquitin-specific proteases (USP) domain of CYLD dictates specificity for cleavage of M1 and K63 linkages (Komander et al., ... 2016), which led to neonatal-onset fever, skin rashes, auto-antibody production, diarrhoea, arthritis and failure to thrive. Both counterstained with DAPI (in blue). Summaries for HIRA gene (According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL) About This Section: Entrez Gene summary for HIRA Gene: This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lys 48 of one Ubiquitin molecule and the carboxy-terminus of a neighboring Ubiquitin molecule. Download Full PDF Package. The FK2 antibody works great in both human and rat samples (I haven't tried mouse) by western blot and immunofluorescence. Cat.No. Not Intended for Diagnostic or Therapeutic Use. 15857-1-AP. Primary Antibody Dilution Buffer: 1X TBST with 5% BSA; for 20 ml, add 1.0 g BSA to 20 ml 1X TBST and mix well. - Find MSDS or SDS, a … 1 Product Result | Match Criteria: Product Name, Description Synonym: Ub Product # Clonality Application Species Reactivity Citations ZRB1494 , 2N21, monoclonal recombinant monoclonal FACS, ICC, WB, affinity binding assay, inhibition assay human pricing. Signal-Seeker tools for studying ubiquitinated proteins, which include Ubiqutin Monoclonal Antibody for detecting mono-ubiquitin, poly-ubiquitin and free ubiquitin, tested in western blot, immuno-fluorescence, immuno-cytochemistry, immuno-precipitation, immuno-cell sorting, applications with proven sensitivity, selectivity, and validated PTM antibodies, also ubiquitin affinity beads and signal seeker ubiquitination kits which are comprehensive tools made from ubiquitin … From: $199. Centrifuge the suspended cells at 1250-1500 rpm/350-300 x g for 5 minutes and decant the buffer. Tested In IHC, ELISA. The Mono- and polyubiquitinylated conjugates, mAb (FK2) Antibody was generated using Ubiquitin as the antigen. Synonym: Anti-CCR4-NOT transcription complex subunit 4 antibody produced in rabbit, Anti-CCR4-associated factor 4 antibody produced in rabbit, Anti-E3 ubiquitin protein ligase CNOT4 antibody produced in rabbit, Anti-Potential transcriptional repressor NOT4Hp antibody produced in rabbit Remove any unbound antibody by washing the cells in 2 mL Flow Cytometry Staining Buffer (Catalog # FC001). Suggested working dilutions are given as a guide only. You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts, Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. IP and WCL were analyzed by immunoblots with αâIKKα antibody (D). monoubiquitin (plural monoubiquitins) (biochemistry) A single ubiquitin moiety, especially when attached to a protein via monoubiquitination Complement component C4c monoclonal antibody (99-72-18) Epithelial sodium channel (γ-subunit, neoepitope) monoclonal antibody (11-35-1) Factor XII (human, heavy chain) monoclonal antibody (10-11-37) Factor XII (human, heavy chain) monoclonal antibody (10-11-5) (biotin conjugate) Factor XII (human, heavy chain) monoclonal antibody (10-11-5) Discover all new products . The antibody does not recognize free ubiquitin. From: $199. This antibody should recognize the … raised against amino acids 1-76 representing full length Ubiquitin of human origin. Chemicals. English [] Etymology []. Choose from 1 of 149 Histone H3 antibodies, which have been validated in experiments with 163 publications and 83 images featured in our data gallery. WT and KDF1 KO keratinocyte (right panels) or cells transfected with plasmid encoding exogenous IKKα (left panels) were treated with MG132 at 10 μM for 6 h and then were subjected to immunoprecipitation using antiâubiquitin (Ub) antibody. Isotype IgG1, kappa. Anti-Ubiquitin Antibody (A-5) is recommended for detection of Ubiquitin and polyubiquitin of mouse, rat and human origin by WB, IP, IF, IHC (P) and ELISA. Items are grouped and shipped together whenever Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec), alamarBlue Cell Proliferation Calculators, This product has been reported to work in the following applications. For proteolysis to occur, Ubiquitin oligomers must be assembled. You cannot modify any Cart contents. possible, and a separate shipping charge will be included for each shipping method required. Tested In IHC, ELISA. We recommend Chrome, Firefox, Opera or Safari to browse our website as they fully support all of our features. Browse primary antibodies for WB, Flow, IHC, ICC/IF, ELISA, IP, and other applications. Formats Unconjugated. Find the Histone H3 antibody that fits your needs. Ubiquitin, as the name implies, is a ubiquitously expressed and highly conserved protein of 8.6 kDa. MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10201-2-AP (ubiquitin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. * *It is possible for a substrate to be both poly-ubiquitinated and multi-mono-ubiquitinated. In particular, mono-ubiquitination by the ubiquitin ligase RAD18 is crucial for Polη-mediated TLS. Buffer. This antibody reacts with human, mouse, rat samples. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Items in Cart (0) Quick Order . From: $199. Histone H2B antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF),Enzyme-linked Immunosorbent Assay (ELISA) applications. We have generated a monoclonal antibody (mAb) that … Reactivity Human. Antibodies. Video Protocols. Isotype IgG2c, kappa. This antibody stains the periphery of senile plaques and of neurofibrillary tangles in Alzheimer's disease brain, the Lewy bodies in Parkinson's disease brain, and the Mallory bodies in alcoholic liver disease. Ubiquitination is a post-translational modification (PTM) that involves the covalent attachment of an 8 kDa ubiquitin (Ub) peptide to one or more lysines of a target protein. Our Dako brand of high-quality diagnostic antibodies, reagents, instruments, software and expertise help hospitals and research labs make accurate tissue-based cancer diagnoses and determine the most effective treatment for cancer patients. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody (ab7780) This image is courtesy of an anonymous Abreview. view now . This paper. nr UBA01-beads) are optimized ubiquitin binding domain affinity reagents that effectively capture endogenous mono- and poly-Ubiquitinated target proteins. Enhancing proteasome activity is one me You can create and edit multiple shopping carts, Edit mode – allows you to edit or modify an existing requisition (prior to submitting). With myc antibody the ubiquitin modified protein might remain undetected because you are mostly detecting the non-modified form. The antioxidant transcription factor Nrf2 confers broad cytoprotection and has a dual role in tumorigenesis. Reactivity Human. (B) Pathway denoted by red arrows illustrates ubiquitin-regulated cell localization of a protein. Product #. Mix well then add 0.1 g BSA , mix. Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. Custom Antibody Labeling We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor ® dyes, DyLight ® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Anti-Mono and Polyubiquitylated Conjugates mAb (FK2) HRP-linked Antibody Activity Assay: The activity of the anti-mono and polyubiquitylated conjugates mAb (FK2) HRP-linked antibody was tested for its ability to recognise mono and polyubiquitin conjugates (Lanes 2 and 3) but not free ubiquitin (Lane1). Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (2). Ubiquitin (Autophagy Marker) MonoSpecific Antibody. Phos. Skip to main content. Ubiquitylation (also known as ubiquitination or ubiquitinylation) is an enzymatic post-translational modification in which a ubiquitin protein is attached to a substrate protein. Determine Ubiquitin Chain Linkage. Query the antibody database by target antigen, species reactivity, host, conjugate or application. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of Ubiquitin and lysine amino groups on the acceptor protein. Mouse anti mono- and polyubiquitin antibody, clone FK2 recognizes both mono- and polyubiquitin chains. Synonym: Anti-ARF-BP1 antibody produced in rabbit, Anti-ARF-binding protein 1 antibody produced in rabbit, Anti-E3 ubiquitin-protein ligase HUWE1 antibody produced in rabbit, Anti-HECT, UBA and WWE domain-containing protein 1 antibody produced in rabbit, Anti-Mcl-1 ubiquitin ligase E3 antibody produced in rabbit, Anti-Mule antibody produced in rabbit, Anti-URE-B1 antibody … This Histone H2B ubiquityl Lys120 (H2BK120ub1) monoclonal antibody from Active Motif has been validated for use in Chromatin Immunoprecipitation (ChIP) and Western blot. Fully-automated and semi-automated system options are available utilizing advanced direct label technology to meet the throughput needs of ⦠Ubiquitin Antibody (A-5) is a mouse monoclonal IgG 2b κ, cited in 61 publications, provided at 200 µg/ml. Search results for ubiquitin antibody at Sigma-Aldrich. Ubiquitin (Autophagy Marker) MonoSpecific Antibody. Many such cases have shown immunoreactivity with the FUS antibody, but none of the FTLDâFUS cases had FUS gene mutations (Urwin et al., 2010). See Abreview. View our standard lab chemicals including Buffers, Stains, Dyes & Probes and much more. (Multi Species Adsorbed), Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed), Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed), Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed), Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed), Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed), Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed), Goat anti Mouse IgG/A/M:HRP (Human Adsorbed), Rabbit F(ab')2 anti Mouse IgG:Dylight®800, Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument, Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility, Select the lasers and filters you wish to include, Select combined or multi-laser view to visualize the spectra. The final step of the ubiquitination cascade requires a ubiquitin-ligating enzyme (E3), which catalyses the covalent attachment of the ubiquitin to its substrate. Please refer to references indicated for further information. Ubiquityl-Histone H2B antibody (Clone 56) - ChIP, WB Ubiquitin is a 76-amino acid globular protein that is highly conserved and stable. Efrat Shema - The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression. 21 Depletion of ubiquitin has been associated to cycloheximide-induced toxicity in Saccharomyces cerevisiae cells. Incubated with the primary antibody for 16 hours at 4°C in 1X PBS/Tween, 5% milk. Reactivity Human. mono-+â ubiquitinNoun []. 50 Cycloheximide treatment reduces total levels of ubiquitin … This process most commonly binds the last amino acid of ubiquitin (glycine 76) to a lysine residue on the substrate. This method is used for less temperature sensitive items such as lab ware and animal Neri Minsky - Monoubiquitinated H2B is associated with the transcribed region of highly expressed genes in human cells. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Learn about the mitochondrial fusion-division cycle and how aberrant regulation results in disease, Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. All lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution Lane 1 : ARPE-19 whole cell lysate - non-transfected Lane 2 : ARPE-19 whole cell lysate - transfected with rRab27a-His Lysates/proteins at 20 µg per lane. However, identifying such modifications in living cells is complex and cumbersome. 1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human ubiquitin (10201-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and … ab7780 staining ubiquitin in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Formats Unconjugated. Using anti-ubiquitin antibody might help (FK2 antibody … Cited in over 360,693 publications. IRS-2 but not IRS-1 was reported to be modified by Nedd4 through mono-ubiquitination to enhance IGF-1 signaling . Please amend your browser settings to enable third-party cookies and access this website’s full functionality. The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA, LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences, Click on image or enlarge button to enlarge, MONOCLONAL MOUSE ANTI-RABBIT/GOAT SECONDARY ANTIBODIES, POLYCLONAL GOAT/RABBIT ANTI-MOUSE SECONDARY ANTIBODIES, ISOTYPE SPECIFIC MOUSE IgG BINDING PROTEINS, PROTEIN A, G-PLUS & L AGAROSE IP REAGENTS, NUCLEOTIDES, PEPTIDES, PROTEINS & AMINO ACIDS, AUTORADIOGRAPHY FILM, TAPE AND PLASTIC WRAP, recommended for detection of Ubiquitin and polyubiquitin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. A short summary of this paper. Ubiquitin Antibody (A-5) is a mouse monoclonal IgG 2b κ, cited in 61 publications, provided at 200 µg/ml; raised against amino acids 1-76 representing full length Ubiquitin of human origin; Anti-Ubiquitin Antibody (A-5) is recommended for detection of Ubiquitin and polyubiquitin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA Interestingly, these Ubiquitin (Ub) affinity beads do not have heavy and light chain contamination, leading to simple and clear data interpretation as seen in the … lab supplies. Biochemical Journal, 2009. Biotinylated ... PCNA protein expression is a well-accepted marker of proliferation. Secondary All lanes : HRP-conjugated goat anti-mouse IgG polyclonal at 1/5000 dilution Developed using the ECL technique. loc_en_US, sid_166553, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)], raised against amino acids 1-76 representing full length Ubiquitin of human origin, Anti-Ubiquitin Antibody (A-5) is recommended for detection of Ubiquitin and polyubiquitin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA, Anti-Ubiquitin Antibody (A-5) is available conjugated to. Domagoj Vucic. ubiquitin æä½ Antibody 10201-2-AP has been identified with IHC, WB,ELISA. Recommended Fluorochrome-conjugated Anti-Rabbit secondary antibodies: Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 Trypsin cleavage of a ubiquitin-conjugate… Primary Antibodies. Antibody Dilution Buffer (1X PBS / 1% BSA / 0.3% Triton X-100): To prepare 10 ml, add 30 µl Triton™ X-100 to 10 ml 1X PBS. Product datasheet. Shipping charges listed below are from our US warehouses to the Contiguous US, Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human histone H2B protein in which Lys120 is mono-ubiquitylated. Search all biomedical databases provided by the National Center for Biotechnology Information (NCBI), an agency of the U.S. National Library of Medicine at the NIH Application. Ubiquitin (Autophagy Marker) MonoSpecific Antibody. The antibodies BML-PW8805 (clone FK1) and BML-PW8810 (clone FK2) are specific for ubiquitin-protein conjugates and show no reactivity with free ubiquitin. Four genes in the human genome code for ubiquitin: UBB, UBC, UBA52 and RPS27A.. Ubiquitin is a small (8.6 kDa) regulatory protein found in most tissues of eukaryotic organisms, i.e., it is found ubiquitously.It was discovered in 1975 by Gideon Goldstein and further characterized throughout the late 1970s and 1980s. Modification of proteins by the addition of lysine (K)-63-linked polyubiquitin (polyUb) chains is suggested to play important roles in a variety of cellular events, including DNA repair, signal transduction, and receptor endocytosis. Compare antibodies from different companies side by side to find the one that best suits your research needs. This usually forms an isopeptide bond between the ε-amino group of a lysine residue within the substrate and the carboxyl group of the C-terminus of ubiquitin ( Hershko and Ciechanover, 1998 ) (see poster). COVID-19 Research Tools; Antibodies. PCNA is mono-ubiquitinated at Lys164 in response to DNA damage at stalled replication forks. Synonym: Anti-CCR4-NOT transcription complex subunit 4 antibody produced in rabbit, Anti-CCR4-associated factor 4 antibody produced in rabbit, Anti-E3 ubiquitin protein ligase CNOT4 antibody produced in rabbit, Anti-Potential transcriptional repressor NOT4Hp antibody produced in rabbit. Tested In IHC. Shipping charges for countries outside the US and Canada will be determined once order has been received, Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. Results TRAF4 binds IRS-1 and promotes its ubiquitination. Monoubiquitination: A dynamic tag for protein regulation. 53. However, identifying such modifications in living cells is complex and cumbersome. This Anti-Ubiquitinylated proteins Antibody, clone FK2 has been published and validated for use in ELISA, Immunofluorescence (IF), Immunoprecipitation (IP) and Western Blotting (WB) for the detection of Ubiquitinylated proteins. 22 Disruption in ubiquitin homeostasis resulting in a decrease in free ubiquitin pools has been implicated in neurodegeneration. Cytoskeleton Inc.’s Affinity beads (Cat. Sodium azide is highly toxic. Definition from Wiktionary, the free dictionary. Autoinhibition of the UBD-containing protein regulates receptor endocytosis and signal transduction (e.g., EGFR recycling 7). This Histone H2B ubiquityl Lys120 antibody was raised against a branched peptide corresponding the the ubiquitin conjugation site at Lys120 of human Histone H2B. Bio-Rad-Antibodies.com relies on third-party cookies to show you pricing, allow you to order online, and connect you to My Bio-Rad. Compare your Western blot to figure 3 above to determine if your substrate is poly-ubiquitinated or multi-mono-ubiquitinated. View our Guide to Histone Modifications and Biological Function. For general protocol recommendations, please visit the, Clinical Diagnostic Antigens and Antibodies, Custom Recombinant Monoclonal Antibody Generation, Custom Service Packages, Pricing, and Ordering, Application Resources and Technical Support, Rapid Custom Antibody Generation for SARS-CoV-2 Assay Development, Anti-Biotherapeutic Antibodies Quality Control and Characterization, Characterization of Critical Reagents for Ligand Binding Assays, Recombinant Fully-Human Immunoglobulin Isotype Controls, PrecisionAb Antibodies - Enhanced Validation for Western Blotting, Antibody Manufacturing to ISO 9001 Quality Assurance Standards, Supports Flow Cytometry, Fluorescence Microscopy and Western Blotting, Multicolor Panel Builder for Flow Cytometry, Articles, Mini-reviews, Educational Summaries, Goat anti Mouse IgG (H/L):Alk. Figure 1: Schematic of ubiquitin-mediated signaling and trafficking. Creative Diagnostics manufactures and markets worldwide the highest quality; innovative; specialty immunoassays. The primary antibodies used for immunofluorescence were: rabbit monoclonal anti-K48 ubiquitin chain (clone Apu2) (Millipore), rabbit monoclonal anti-K11 ubiquitin chain (clone 2A3/2E6) (Millipore), rabbit monoclonal anti-K63 ubiquitin chain (clone Apu3) (Millipore) and mouse monoclonal anti-K63 ubiquitin chain antibody (clone HWA4C4) (Millipore), rabbit polyclonal anti-GFP-HRP antibodies (Rockland), mouse monoclonal anti-ubiquitin-HRP antibody … Anti-Ubiquitinylated proteins Antibody, clone FK2: Background Information: Ubiquitin exists either covalently attached to another protein, or free (unanchored). Background. We’ve detected that you are using Internet Explorer. The FUS protein is involved in DNA repair and regulation of RNA splicing (Seelaar et al., 2010). Note: If an unconjugated primary antibody is used, incubation with an appropriate secondary antibody should occur now. ãçç£ç¾å ´ãé£åè¡çãªã©å¹ åºãåéã«350ä¸ç¹ä»¥ä¸ã®åæãã§ãå¿ãããååãµã¤ãã3000å以ä¸ã注æã§éæç¡æã Anti-p-Ubiquitin (pSer65) Antibody, clone 2N21 ZooMAb ® Rabbit Monoclonal. Anti-Ubiquitin antibody produced in rabbit. (A) Pathway denoted by black arrows illustrates ubiquitin-driven autoinhibition. Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184) LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Distinguish Between Poly-ubiquitination and Multi-mono-ubiquitination. Download PDF. view now. Here we report that TRAF4, a RING domain E3 ubiquitin ligase, mediates non-conventional ubiquitination of IRS-1 to promote its function. Jump to navigation Jump to search. Background The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin.
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